FIG 1.

Mutants with mutations in Brl1 are temperature sensitive and affect mRNA export and NPC assembly and distribution. (A) Temperature sensitivity of brl1 mutant strains. Cells were grown at 30°C in YPD, and serial dilutions were spotted onto YPD plates. (B) Mutations in Brl1 affect mRNA export. Cells were incubated at the indicated temperatures for 18 h, and the subcellular distribution of poly(A)⁺ mRNA was analyzed by in situ hybridization. DNA was stained with DAPI. (C) Brl1 affects the distribution and assembly of the NPC. The distribution of the nuclear basket protein Nup60-GFP and that of Nup82-GFP, a component of the cytoplasmic filaments, were analyzed by fluorescence microscopy. Bar, 5 μm.