FIG 3.

brl1 mutant cells are hypersensitive to the membrane-fluidizing agents oleic acid and benzyl alcohol. (A) Hypersensitivity of brl1 mutant cells to membrane-fluidizing agents. Strains were grown in YPD; 10-fold serial dilutions were spotted onto plates containing the indicated concentration of oleic acid or BA; and plates were incubated at 30°C. The hypersensitivity of brl1 and brr6 mutant strains to these agents is indicative of hyperfluid membranes and deregulation of membrane composition. (B) Mutants lacking the capacity to synthesize neutral lipids are hypersensitive to BA. The indicated strains were cultured in YPD, diluted, and spotted onto plates containing the indicated concentration of BA.