FIG 5.
Adaptive changes in lipid profiles of wild-type and brl1 mutant cells upon treatment with benzyl alcohol. Wild-type cultures grown at 24°C in SC medium were split, diluted to an OD600 of 0.25, and either left untreated or treated with a sublethal dose of BA (0.2%). Cultures with equal ODs were harvested at the indicated time points; lipids were extracted; and fatty acids and sterols were analyzed. Wild-type cells treated with BA show lower levels of the short-chain C14 fatty acids myristic acid (A) and myristoleic acid (B), with concomitant increases in the levels of the long-chain C18:0 saturated fatty acid stearic acid (C) and ergosterol (D). These changes are probably induced to counteract the fluidizing effect of BA. (E and F) Recovery of wild-type and brl1-1 mutant cells from treatment with BA. Cells were cultivated at 24°C and were treated with BA (0.2%) for 5 h. Cells were washed with fresh medium and were recultivated for another 5 h or 10 h. Lipids were extracted, and fatty acid profiles were recorded by GC and were plotted relative to the levels present at the zero time point. Values are means ± standard deviations for three independent experiments.