FIG 4.

Cell adhesion by strain tetR-SEC15. Gene expression was repressed by growing cells for 3 h in YPD with or without DOX, and then cell adhesion was assayed in RPMI buffered to pH 7.0 with 50 mM MOPS supplemented with l-glutamine for another 2 h, with or without DOX; metabolic activity was measured using the XTT reduction assay. Under repressing conditions, adhesion of cells was significantly decreased in strain tetR-SEC15 grown with DOX compared to that in strain tetR-SEC15 grown without DOX. The asterisk denotes a statistically significant difference (P < 0.05).