Enzymes are crucial for fungal pathogenesis and can alter the infection process. These enzymes are potential targets for new antifungal agents. (A) Some pathogenesis-related enzymes are retained to be active inside the cell body, while others are secreted. Some, like laccase, are both retained and secreted. (B) Of those released, some are secreted using traditional secretion systems, while others are included as cargo in extracellular vesicles.