TABLE 3.

Possible target enzymes for rational antifungal drug design

Enzyme(s)	Comment(s)	Reference(s)
14α-Demethylase	A critical enzyme in sterol assembly	119
Glucosylceramide synthase	Glucosylceramide plays critical role in pathogenicity of C. neoformans	127, 128
Laccase	Melanization aids virulence	60, 63, 64, 65
Myristoyltransferase	Myristoylation inhibition is fatal for C. neoformans	116, 117
Phosphoribosylaminoimidazole carboxylase	Mutants that cannot synthesize adenine have reduced virulence	114
Pyrophosphorylase and cytosine-specific permease	Enzymes are basis of C. neoformans flucytosine resistance	201, 202
Sterol synthesis enzymes	Sterol synthesis enzyme mutants show resistance to fluconazole and amphotericin	122–124