FIG 8.

AbA causes the accumulation of neutral lipids. (A) Exponentially growing WT and ypc1Δ ydc1Δ (yyΔΔ) cells were extracted directly or further incubated for 4 h with 0.25 μg of AbA. Lipid extracts were analyzed by MS. Results are expressed as the percentage of lipids identified in positive (SE, TAG, and PE) or negative (PS and PI) ion mode. Error bars for two technical replicates are shown. Only lipids that change with AbA treatment are shown. Results are from one of two experiments giving the same result. (B and C) Tenfold dilutions of exponential-phase WT and pah1Δ lpp1Δ dpp1Δ cells (B) or tenfold dilutions of exponential-phase cells carrying deletions in 3 of the 4 enzymes making sterol esters (SEs) and TAGs (ARE1, ARE2, DGA1, and LRO1) and having the promoter of the genomic copy of the fourth gene, either DGA1 or LRO1, replaced by the GAL1 promoter (C) were plated onto YPD or YPGal with the indicated concentrations of AbA and incubated for 2 (B) or 3 (C) days at 30°C.