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. 2015 Dec 1;5:17507. doi: 10.1038/srep17507

Figure 2. Treatment information of the subject and the trunk-branch genetic structure of the three sub-clones.

Figure 2

(A) the first arrow shows treatment regimens, H, Isoniazid; R, Rifampicin; Z, Pyrazinamide; E, Ethambutol; AMK, Amikacin; LFX, Levofloxacin; PAS, Para-aminosalicylic acid. Drugs in green represent effective drugs. The second arrow contains the sputum smear microscopy results at each time point. ‘−’ represents smear negative; a number (such as 5, 8) represents the actual bacilli number that observed in 300 visual fields; ‘+’ represents 3 ~ 9 bacilli per 100 visual fields; ‘++’ represents 1 ~ 9 bacilli per 10 visual fields. The pie charts refer to the sub-clones identified through single colonies genotyping and the percentage of each chart represents the relative frequency of each sub-clone detected SNPs in deep sequencing data. (B) detailed phylogeny of the three sub-clones with common SNPs mapped to the trunk and heterogeneous SNPs mapped to the branches. Drug-resistant mutations are shown in bold and parallel mutations are highlighted in green. murA-rrs (M1) refers to SNP 1471660 G-C while murA-rrs (M2) refers to SNP 1471656 G-C. Rv0749 (M3) and Rv0749(M4) refer to two different SNPs in Rv0749.