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. 2015 Dec 1;5:17604. doi: 10.1038/srep17604

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(A) Hepatic fatty acid composition for total lipids, (B) the cholesterol ester (CE) fraction, (C) the triglyceride (TG) fraction and (D) the phospholipid (PL) fraction in livers from Elovl6^+/+Ldlr^−/− and Elovl6^−/−Ldlr^−/− mice fed a standard diet (SD) or a lithogenic diet (LD) for 4 weeks. Hepatic total lipids were extracted, and the major classes of lipids were separated on a silica column. The lipid fractions were methyl-esterified and quantified by gas chromatography (n = 3–6 per group). ^*P < 0.05, ^**P < 0.01.