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. 2015 Dec 1;5:17604. doi: 10.1038/srep17604

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Elovl6^+/+Ldlr^−/− and Elovl6^−/−Ldlr^−/− mice were fed a standard diet (SD) or a lithogenic diet (LD) for 4 weeks and sacrificed following 4 h of food deprivation (n = 8–13 per group). (A) qPCR analysis of genes for BA metabolism. ^*P < 0.05, ^**P < 0.01. (B) Immunoblot analysis of CYP7A1, CYP27A1, SLC10A1 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in the livers of Elovl6^+/+Ldlr^−/− and Elovl6^−/−Ldlr^−/− mice fed a SD or a LD for 4 weeks.