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. 2015 Dec 1;5:17404. doi: 10.1038/srep17404

Figure 1. Pharmacokinetics of 35 K-Fc following ip injection.

Figure 1

5 μg of 35 K-Fc was injected ip into C57bl/6J mice. The peritoneal cavity was lavaged with PBS and the plasma prepared from mice harvested over 7 days following injection (n = 3 per timepoint). The concentration of 35 K-Fc in peritoneal lavage, calculated as total remaining per animal (A), and plasma concentration (B) was determined by ELISA. The bioactivity of 35 K-Fc was determined using a chemotaxis bioassay. Plasma (10%) was used as a chemoattractant in a Boyden Chamber chemotaxis assay. The number of cells migrating towards the plasma samples was calculated as a migration index normalized to the amount of random migration seen to buffer alone (C). The concentration of RANTES in the plasma 5 was measured by ELISA (D). The plasma 35 K-Fc concentration was correlated with the plasma bioactivity (E). n = 3 per timepoint, Statistical analysis performed by one-way ANOVA and Dunnett’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001 compared to untreated. Linear correlation assessed by Spearman’s Test.