Skip to main content
. 2015 Dec 1;5:17404. doi: 10.1038/srep17404

Figure 3. R89A 35 K-Fc expression following hydrodynamic delivery causes significant suppression of zymosan induced peritonitis.

Figure 3

Mice were injected with 1 μg 35 K-Fc R89A 35 K-Fc plasmid or GFP control plasmid 5 days prior to injection of 100 μg zymosan ip (A). 16 hours after the injection of zymosan recruited cells were recovered by peritoneal lavage and plasma samples harvested for ELISA. Animals receiving R89A 35 K-Fc plasmid expressed 35 K-Fc in the plasma, whereas the protein was not detected in the GFP control animals (B). Leukocytes reovered from the peritoneal cavity were stained with 7/4 and Ly-6G antibodies to detect recruited monocytes and neutrophils (C). Injection of zymosan caused recruitment of both monocytes and neutrophils, but the number of cells recruited was significantly reduced in animals that received R89A 35 K-Fc (D,E). *p < 0.05. Statistical test performed by T-test.