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. 2015 Dec 1;5:17395. doi: 10.1038/srep17395

Figure 5. Increased acetylation of GKRP in db/db mice.

Figure 5

(A) Western blot of acetylated GKRP in db/db mouse livers. Cell lysates were immunoprecipitated by an anti-GKRP antibody and immunoblot performed to detect endogenous acetylated GKRP using an anti-Ac-Lys antibody. (B) Band intensities of acetylated GKRP, as quantified by Image J software. The values from db/m + mice were set to 1.0 (n = 8). (C) Interactions between GK and GKRP in control and db/db mice. Endogenous GKRP was immunoprecipitated from liver homogenates of db/m + and db/db mice, using GKRP antibody, and then immunoblotted by an anti-GK antibody. (D) Band intensities of the GK-GKRP complexes were quantified using Image J software. The values from db/m + mice were set to 1.0 (n = 4). Values are expressed as means ± SEM, *p ≤ 0.05.