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. 2015 Nov 19;7(11):5933–5961. doi: 10.3390/v7112915

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Slow-maturing DsRed does not accurately reflect viral protein expression. PK15 cells were infected with pseudorabies virus (PRV) strains expressing EGFP-tagged VP22 (left column), or DsRed-tagged VP22 (right column), and imaged at 4, 6, and 8 hpi. Because of its fast fluorophore maturation (EGFP t_1/2 = tens of minutes), EGFP-tagged VP22 can be readily detected by confocal microscopy at 4 h post-infection (hpi). DsRed-tagged VP22 is not efficiently detected throughout the course of infection likely due to slow fluorophore maturation (DsRed t_1/2 = ~10 h). Scale bars represent 10 μm.