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CAR mRNA expression in macrophages. Monocytes and MDM treated with M-CSF or GM-CSF for seven days were collected, and RNA was extracted. CAR mRNA and β-actin were amplified using real-time RT-PCR. CAR relative expression was determined using the 2−ΔΔCt formula, as compared to parental monocytes. These results are the mean ± SEM of three independent experiments.
