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. 2015 Dec;25(12):1848–1859. doi: 10.1101/gr.195404.115

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© 2015 Janich et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genome Research, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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Figure 3. — Core clock transcripts show mRNA abundance and ribosome occupancy in sync. (A) Time-resolved distribution (ZT00 to ZT22; arranged vertically) of normalized counts of ribosome profiling (RPF-seq; blue) and RNA abundance (RNA-seq; orange) reads along the Arntl/Bmal1 and Nr1d1/Rev-erb alpha transcripts. Different color shadings (dark/light orange and blue) indicate the two biological replicates. Gray shading of the boxes marks the CDS; UTRs are in white. (B) RPKM values of CDS-mapping RPF-seq (blue) and RNA-seq (orange) data for circadian core clock genes around the 24-h daily cycle. Means per timepoint are plotted; error bars, the two biological replicates. Dashed lines represent rhythmic curve fittings to the data.