Figure 10.
Carbon nanotube-polymer composite (CNP) for ex vivo T cell expansion. (a) Schematic of CNP assembly. Neutravidin-adsorbed CNTs (NCNT) are associated with biotinylated T cell stimuli and biotin-presenting PLG nanoparticles encapsulating IL-2 and magnetite. (b) Schematic of CNP functionality. CNPs present high density surface-adsorbed T cell stimuli, facilitate paracrine IL-2 release, and can be separated from T cells using magnetic separation. (c) Flow cytometry plots showing enhanced expansion of human EBV-specific CD8+ human T cells using CNPs presenting EBV peptide in MHC or anti-CD3 (OKT-3), compared to primary DCs loaded with EBV peptide and cultured in soluble IL-2. From [144].