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. 2015 Oct 7;10(6):3393–3398. doi: 10.3892/ol.2015.3782

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Copyright: © Kong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — (A) The fluorescence emission of thyroid carcinoma K1 cells transfected with control siRNA and RBP2-siRNA was recorded (magnification, ×200). (B) The protein expression levels of RBP2 were lower in the RBP2-siRNA-transfected cells than in the blank and negative control cells, according to the results of immunocytochemical analysis (magnification, ×400). (C) Transwell assay revealed the presence of invasive cells through the membrane in the control and RBP2-siRNA groups (magnification, ×200). (D) The cell migration ability was lower in the RBP2-siRNA-transfected cells, compared with the control cells, as evaluated by transwell assay (magnification, ×200). (E) Western blot analysis demonstrated that the protein expression levels of RBP2 were markedly reduced in the RBP2-siRNA group, compared with the blank and negative control groups. β-actin was used as reference. siRNA, small interfering RNA; RBP2, retinoblastoma binding protein 2; RBP2-siRNA, siRNA targeting RBP2; blank-group, non transfected cells.