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. 2015 Oct 7;10(6):3393–3398. doi: 10.3892/ol.2015.3782

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Copyright: © Kong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — (A) Immunocytochemical and (B) RT-qPCR analyses revealed a marked difference in the (A) protein and (B) mRNA expression levels of RBP2 in thyroid carcinoma K1 cells transfected with RBP2-siRNA, compared with the control cells. (C) Western blot analysis demonstrated a significant difference in the protein levels of RBP2 across the 3 groups of cells. β-actin was used as reference. Transwell assay identified a lower number of (D) invasive and (E) migrated cells in the RBP2-siRNA-transfected cells, compared with the control group. (F) The transfection with RBP2-siRNA affected the tumorigenicity ability of K1 cells, since a lower number of newly formed colonies were observed in the RBP2-siRNA-transfected group, compared with the control group. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; RBP2, retinoblastoma binding protein 2; siRNA, small interfering RNA; RBP2-siRNA, siRNA targeting RBP2; control siRNA, negative control siRNA targeting RBP2; blank group, non transfected cells. **P<0.01 vs. blank group, negative control; *P<0.05 vs. negative control.