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. 2015 Dec 1;5:17594. doi: 10.1038/srep17594

Figure 2. Identification of Id4-2A-CreERT2-2A-tdTomato knock-in mice.

Figure 2

(a) Fluorescence image of frozen sections of Id4-creERT2-tdTomato mouse testes. Immunofluorescence staining shows the Id4 protein was coexpressed with tdTomato in the testes of Id4 knock-in mice. (b) Left panel, Representative scatter plots of tdTomato+ and tdTomato− populations isolated from the testes of 8-day-old Id4 knock-in mice(n = 5), determined using fluorescence-activated cell sorting (FACS). Right panel, The levels of Id4, Cre and Pax7 mRNAs were determined by real-time PCR in the tdTomato+ population compared with tdTomato− population. The value from the tdTomato+ sample was set as 1. GADPH served as an internal control. (c) Expression of lacZ is detected only in Id4+ cells of Id4-CreERT2-tdTomato; Rosa26- flox-stop-LacZ mice using X-gal staining, 2 days after TM induction. Whole mount staining for GFRA1 after X-gal staining showed that these lacZ+ cells are also GFRA1+ undifferentiated spermatogonia. Scale bar, 50 μm.