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Uptake of ALA in the esophageal squamous cell cancer cell line. ALA-induced PPIX red fluorescence was not observed in TE-1 cells incubated (A) without ALA, but was observed in TE-1 cells incubated with (B) 0.25, (C) 0.5 and (D) 1.0 mg/ml ALA. Original magnification, ×100. ALA, 5-aminolevulinic acid; BF, buffered formalin; PPIX, protoporphyrin IX.
