Effect of RAPA on the cell cycle of osteosarcoma cells. Osteosarcoma cells (MG63 and U-2-OS) were infected with miR-126-overexpressing or -silencing lentiviral vectors. The effect of RAPA on the cell cycle of the osteosarcoma cells was then observed by flow cytometry. Blank control, untreated MG63 and U-2-OS. DDP, cisplatin; MTX, methotrexate; RAPA, rapamycin; pre-miR-126, osteosarcoma cells infected with miR-126-overexpressing lentiviral vectors; anti-miR-126, osteosarcoma cells infected with miR-126-silencing lentiviral vectors. ▲▲P<0.01v.s. untreated control group; ΔΔP<0.01 v.s. pre-126 group; #P<0.05 v.s. RAPA group.