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. 2015 Sep 22;10(5):1959–1966. doi: 10.3892/etm.2015.2763

Figure 4.

Figure 4.

Effect of LW6 on HIF-1α, GLUT1 and certain enzymes involved in glucose metabolism in activated T cells. (A) Stimulation of T cells increased the levels of the transcription factor HIF-1α, whereas treatment with LW6 decreased HIF-1α levels. A similar pattern was observed in the case of (B) GLUT1, (C) HKII and (D) LDH-A. (E) Stimulation of T-cells did not affect the level of PDH, whereas treatment with LW6 increased PDH levels. (F) Stimulation of T-cells did not affect the level of pPDH phosphorylated at serine 393, whereas treatment with LW6 returned pPDH to its basal level. HIF-1α, hypoxia-inducible factor-1α; OD, optical density; GLUT1, glucose transporter-1; HKII, hexokinase II; LDH-A, lactate dehydrogenase-A; PDH, pyruvate dehydrogenase; pPDH, PDH phosphorylated at serine 393. Assays were conducted in control (resting), activated and LW6-treated activated T cells.