FIG 4.

Influence of IL-29 pretreatment on rSeV/eGFP growth in WD-PBECs. WD-PBECs (n = 2 to 3 donors) were pretreated with IL-29 (100 or 1,000 pg/ml) or mock treated. Twenty-four hours later, cultures were infected with rSeV/eGFP at an MOI ≈ 0.1. (A) UV microphotographs were taken every 24 hpi. Original magnification, ×4. (B) eGFP expression was quantified every 24 hpi by measuring the percentage of green pixels in three random microscopic fields. Virus growth kinetics were determined by titrating rSeV/eGFP in apical washes at 24-h intervals after infection (C). The data are presented as means ± the SEM log₁₀ FFU/ml. Areas under the curves were calculated and compared by using an unpaired Student t test. *, P < 0.05.