FIG 4.

Disease-associated PrP-res in tg-deer-PRNP mice inoculated with different CWD allotypes. (A) PrP-res from brains of prion-affected tg33 (wt-PrP^C) and tg60 (S96-PrP^C) mice. Brain homogenates were digested with proteinase K (PK) and analyzed by SDS-PAGE and Western blotting. Lanes M, molecular size markers. PrP-res from tg33 mice had similar molecular masses after enzymatic cleavage (A) and equivalent glycoform ratios (B). S96-PrP-res has a lower molecular mass and was detectable only in brain homogenates derived from tg60 mice infected with H95⁺ CWD allotypes. UI, homogenates from tg mice inoculated with uninfected deer brain homogenate. PrP-res detection was achieved with anti-PrP monoclonal antibody BAR224.