FIG 9.

Phenotype of immune evasion functions of Ad6 and Ad26. (A) Surface MHC I (pan-HLA-A, -B, and -C) detection by flow cytometry on A549 cells after mock infection or infection with Ad6, Ad26, or E3-deleted viruses after 6 h of infection and 3 h of MHC I recovery (see Materials and Methods for details). Averages and standard errors for five independent experiments are shown. (B) Surface MHC I (pan-HLA-A, -B, and -C) detection by flow cytometry on A549 cells after 24 h of infection. Averages and standard errors for three experiments are shown. (C) TRAIL-induced cell death and resistance after mock infection or infection with Ad6, Ad26, or E3-deleted virus. Percent viable (propidium iodide-negative [PI⁻] and annexin V-negative) cells plotted are averages of data from three experimental samples. Significance was determined by two-way analysis of variance with a Tukey posttest. Significance at a specific concentration is denoted with a symbol or number next to the asterisk(s), where ****# indicates significance of Ad6 infection compared to mock infection at 0.5 mg/liter and **∞ indicates significance of Ad26 and Ad6ΔE3 infections compared to mock infection at 0.5 mg/liter (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.001).