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Determination of HIV-1 Env expression by capture ELISA. 293T cells were transfected by plasmids expressing wild-type (WT) or mutant Envs. The proteins (gp120/gp41) in the lysates of transfected cells (A) or culture supernatants (B) were captured by sheep anti-gp120 antibody D7324 and detected by human MAbs VRC01 and 10E8.
