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Effects of MTSC22-induced mutations on the conformation of 6-HB structure. The reactivity of 6-HBs formed by N36 and C34 or their mutants with conformation-dependent MAbs NC-1 (A), 17C8 (B), and 2G8 (C) was tested by ELISA. The peptide mixture was used to coat the plate wells at 10 μg/ml, and the final concentration of a tested MAb was 5 μg/ml. Data were derived from three independent experiments and are expressed as means and standard deviations.
