3-D reconstruction of TuMV-induced membrane rearrangement at midstage of infection. (I to IV) Representative tomogram slices generated on a 200-nm-thick section from TuMV-infected vascular parenchymal cell at 6 dpi. The SMVL structures in close proximity to dilated rER and a cytoplasmic inclusion body are shown. (V and VI) A 3-D surface rendering of the closely packed SMTs, dilated rER, and cytoplasmic inclusion body. Red arrows, connection between a SMT and the rER membrane; yellow, SMTs, sky blue, rER; magenta, cytoplasmic inclusion body. CI, cytoplasmic inclusion body; rER, rough endoplasmic reticulum; SMVL, single-membrane vesicle-like structure.