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Western blot analysis of SIRT1 expression in the PC-3 cells transfected with miR-34a. Protein lysates were extracted at 48 h post-transfection. (A) Equal amount of protein was loaded into each well as indicated by the expression of the GAPDH protein. (B) Densitometry quantification of effect of transfection of miR-34a in the PC-3 cells (n=4, ± SD; *P<0.05 vs. negative control).
