Table I.

Characteristics of the primers used for PCR.

Variant	Exon	Primer sequence	Product size (bp)	Tb (°C)
p.I171V		F: 5′-TTATGGATGTAAACAGCCTC-3′
c.511A>G	5	R: 5′-TACCGAACTATAACACAGCA-3′	328	54
c.657-661del		F: 5′-CAGATAGTCACTCGGTTTACAA-3′
c.657_651delACAAA	6	R: 5′-TTCTTTAGGAAAATTTAGCT-3′	228	50
p.R215W		F: 5′-CAGATAGTCACTCGGTTTACAA-3′
c.643C>T	6	R: 5′-ACAACTACTGATAAGAGTTA-3′	273	50

PCR, polymerase chain reaction; F, forward; R, reverse; bp, base pairs; Tb, binding temperature.