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. 2015 Aug 28;10(5):2691–2698. doi: 10.3892/ol.2015.3654

Figure 3.

Figure 3.

miRNA-34a-5p modulated the chemo-sensitivity by targeting AXL. (A) Western blot were performed to detected the expression of AXL in 97L cells transfected with miRNA-34a-5p mimics or inhibitors (P<0.05). (B) The binding situ of wild type and mutant AXL for miRNA-34a-5p. (C) Luciferase Reporter assay were performed to test the relative luciferase activity of wild type or mutant AXL after 97L cell were transfected with miRNA-34a-5p, respectively. (D) Western blot assay were conducted to detect the expression of AXL after 97L cells were transfected with siAXL (P<0.05). (E) Cell proliferation assays were performed to detect the relative changes of cell numbers in 97L cells transfected with siNC or siAXL, respectively (P<0.05). (F) Flow cytometry assay were performed to detect the apoptosis ratio in 97L cells transfected with siAXL with or without pretreatment of cisplatin, respectively (P<0.05). (G) Western blot were performed to detect the expression of apoptosis protein caspase-3 in 97L cells transfected with siAXL with or without pretreatment of cisplatin, respectively (P<0.05). All experiments were performed in triplicate (**P<0.001). miRNA-34a-5p, microRNA-34a-5p; Si-NC, negative control small interfering RNA; Si-AXL, AXL small interefering RNA; WT, wild-type; MUT, mutant.