Table 2.
Sequencing runs and genome assembly details
| Sequencing run | Raw reads | Filtered reads | Read length | Library prep |
| MP1a | 3845734 | N/A | 2 × 80 bp | Nextera Mate-Pair |
| MP2a | 31223454 | 12095792 | 2 × 80 bp | Nextera Mate-Pair |
| MP3a | 14121900 | 4397510 | 2 × 250 bp | Nextera Mate-Pair |
| PE1b | 213604902 | 29314946 | 2 × 250 bp | Nextera XT |
| Genome Assemblyc | Contigs | N50 (bp) | Assembly Size | |
| Mitochondria | 1 | 41,656 | 41,656 | |
| Contigs | 31,194 | 3,411 | 48,938,887 | |
| Scaffolds | 26,811 | 19,415 | 49,120,517 | |
| Gap-fill | 26,811 | 19,012 | 48,939,625 | |
| Final assembly (minus <500 bp, contaminants) | 14,699 | 26,144 | 44,270,879 |
aThree runs of a Nextera mate-pair library of Balamuthia strain 2046 from axenic culture sequenced on an Illumina MiSeq
bNextera XT paired-end library of strain 2046 sequenced on an Illumina HiSeq
cAssembly of mitochondrial genome from MP1 library, of whole-genome from all four libraries