Figure 1.

Time-dependent effects of hydrogen-rich saline on the release of sex hormones (E₂ and FSH) during the process of chemotherapy-induced ovarian injury. Cisplatin was injected twice, each time at 5 mg/kg, in group OI. The same dose of saline was injected in group Con. The rats received intraperitoneal hydrogen-rich saline at 10 ml/kg body weight for 14 days. The levels of (A) FSH and (B) E₂ were measured on the 14th, 28th and 42nd days (T₁, T₂ and T₃) after the cisplatin injection. Results are presented as the mean ± standard deviation (n=10 per group). ^aP<0.05 compared with group Con; ^bP<0.05 compared with group OI. E₂, estrogen; FSH, follicle-stimulating hormone; Con, control; Con + H₂, control + hydrogen-rich saline; OI, cisplatin-induced ovarian injury; OI + H₂, cisplatin-induced ovarian injury + hydrogen-rich saline.