. 2014 Jan 6;3(1):24–37. doi: 10.3390/antiox3010024

Table 2.

Determination of the thiobarbituric acid reactive substances (TBARS), carbonyl protein, superoxide dismutase (SOD), and catalase (CAT) activities in the MRC5 cells pretreated with the extract of bracts of A. angustifolia and H₂O₂.

Treatments	TBARS (nmol TMP/mg of protein)	Carbonyl protein (nmol DNPH/mg of protein)	SOD (U SOD/mg of protein)	CAT (mmol H₂O₂/min/mg of protein)
Cell Control	0.57 ± 0.08 ^a	1.85 ± 0.08 ^a	19.40 ± 0.49 ^b	15.19 ± 0.56 ^a
H₂O₂ 900 µM	2.21 ± 0.06 ^c	4.51 ± 0.15 ^c	9.32 ± 0.13 ^d	9.75 ± 0.08 ^c
AAE 25 µg/mL	0.54 ± 0.01 ^a	1.94 ± 0.10 ^a	19.00 ± 0.34 ^b	14.25 ± 0.65 ^a
AAE 50 µg/mL	0.51 ± 0.15 ^a	1.88 ± 0.06 ^a	23.81 ± 0.01 ^a	14.63 ± 0.06 ^a
AAE 25 µg/mL + H₂O₂	0.86 ± 0.03 ^b	3.09 ± 0.51 ^b	13.46 ± 0.02 ^c	12.38 ± 0.01 ^b
AAE 50 µg/mL + H₂O₂	0.52 ± 0.16 ^a	2.24 ± 0.16 ^a	19.51 ± 0.22 ^b	14.07 ± 0.05 ^a

Data are mean ± SD values. Different letters indicate significant differences according to analysis of variance (ANOVA) and Tukey’s post-hoc test (p ≤ 0.05) in each assay.