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(a) HFA sandwich sandwich enzyme linked immunosorbent immunoassay (ELISA) using various EDC-based strategies for crosslinking anti-HFA antibodies to APTES-functionalized 96-well microtiter plates (MTP); (b) bicinchoninic acid (BCA) protein assay to determine the amount of anti-HFA antibody bound to MTP by various strategies. All experiments were done in triplicate. The errors bars represent standard deviation.
