Table 3.
Polyphenol and flavonoid content, antioxidant and free radical scavenging activities of ethanolic extract of Nardostachys jatamansi (NJE) and the hexane fraction (NJH). Values are represented as the means ± SD of the triplicate determination. ABTS, 2,2′-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) diammonium salt. CE, catechin equivalents.
| Assay | Ethanolic Extract (NJE) | Hexane Extract (NJH) |
|---|---|---|
| Total polyphenolic content | 53.06 ± 2.2 mg GAE/g of extract | 13.87 ± 1.3 mg GAE/g of extract |
| Total flavonoids | 25.303 ± 0.9 mg CE/g of extract | 4.58 ± 0.3 mg GAE/g of extract |
| DPPH radical scavenging assay (IC50) | 222.22 ± 7.4 μg/mL | 432.68 ± 13.7 μg/mL |
| Metal chelation (IC50) | 948 ± 21.1 μg/mL | 1211 ± 27.8 μg/mL |
| ABTS (IC50) | 13.90 ± 0.5 μg/mL | 23.57 ± 1.4 μg/mL |
| Superoxide (IC50) | 113.81 ± 4.2 μg/mL | 255.72 ± 9.7 μg/mL |
| Anti-lipid peroxidation (IC50) | 465.11 ± 14.3 μg/mL (brain) | 587.53 ± 17.6 μg/mL (brain) |
| 539.08 ± 18.9 μg/mL (liver) | 685.15 ± 13.4 μg/mL (liver) | |
| Ferric-reducing antioxidant power | 12.3 ± 0.43 mg FeSO4E/g of extract | 45.62 ± 1.34 mg FeSO4E/g of extract |