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. 2015 Apr 23;5(2):177–188. doi: 10.3390/diagnostics5020177

Figure 3.

Figure 3

Specificity of capture (AB1) and detection (AB2) antibodies: To validate the utility and specificity of the selected antibodies (AB1 and AB2), LPGDS (20 ng) was resolved on a 12% SDS-PAGE, blotted to PVDF membrane, blocked with 3% milk in TBS and probed with AB1 and AB2 culture supernatants. Secondary antibody is HRP labeled goat anti mouse from Jackson Laboratories (1:5000) and HRP labeled goat anti rabbit from Biorad (1:300). Protein bands were detected by ECL (GE Healthcare) with a LICOR C-digit reader (scan time 6 min).