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. 2015 Sep 23;10(6):3619–3624. doi: 10.3892/ol.2015.3737

Figure 1.

Figure 1.

RT-qPCR and western blot analysis of SUSD2 expression in paired NSCLC and adjacent normal lung tissues. (A) Significant differences in SUSD2 mRNA expression between the NSCLC and adjacent normal lung tissues (P<0.0001). (B) Fold-changes (2−ΔΔCt values) detected by RT-qPCR revealed reduced expression of SUSD2 mRNA in the majority of NSCLC cases (20/24), when compared with that of paired normal lung tissues. Expression levels were normalized to β-actin. (C) Western blotting indicated downregulation of SUSD2 protein expression in NSCLC tissues (21/24), compared with that of adjacent normal lung tissues. β-actin was used as an internal control. (D) Significant differences in SUSD2 protein expression between the NSCLC and adjacent normal lung tissues (P<0.0001). RT-qPCR, reverse transcription-quantitative polymerase chain reaction; SUSD2, sushi domain containing 2; NSCLC, non-small cell lung cancer; mRNA, messenger RNA; T, NSCLC; N, normal.