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Examples of Rotor Gene 3000 printouts showing detection of PRDX1mRNA by qPCR assay. (A) Samples of known concentration and tissue cDNA were detected by qPCR. (B) Fluorescent signals of both 10-fold serial dilutions of sample with known concentration and tissue cDNA were detected by qPCR. (C) Melting curves of amplified PRDX1 were plotted. PRDX1, peroxiredoxin 1; qPCR, quantitative polymerase chain reaction; CT, cycle threshold.
