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. 2015 Oct 20;47(6):2073–2081. doi: 10.3892/ijo.2015.3213

Figure 2.

Figure 2

Effect of VPA on the acetylation of histone H3 in SKBR3 cells. (A) Time courses of changes in acetyl-histone H3 and histone H3. SKBR3 cells were treated with 1 mM VPA, and cell lysates were harvested after time courses of ≤48 h. Western blotting was performed using a series of primary antibodies. (C) Time courses of changes in acetyl-histone H3 of SKBR3 cells treated with 100 nM TSA after ≤48 h. (A and C) The amount of β-actin in each sample was used as the loading control. (B and D) The expression levels of acetyl-histone H3 were analyzed by the Light-Capture system and then quantified using the CS analyzer program and reported in the histogram after normalization against β-actin, relative to the values obtained in the absence of VPA (ratio).