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Effect of VPA on the expression of p21 WAF1 in SKBR3 cells with the indicated incubation times. (A) The expression levels of p21 WAF1 were detected by immunoblotting on protein extracts prepared from SKBR3 cells treated with 1 mM VPA for ≤48 h. The amount of β-actin in each sample was used as the loading control. (B) The expression levels of p21 WAF1 were reported in the histogram after normalization against β-actin, relative to the value obtained in the absence of VPA (ratio).
