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. 2015 Sep 25;10(6):2093–2101. doi: 10.3892/etm.2015.2780

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Copyright: © Kong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Impact of PFP storage at −80°C for 1 or 4 weeks on MV counts and size distribution. Impact of (A) heparin and (B) EDTA on MV counts, as well as impact of (C) heparin and (D) EDTA on MV size distribution. Representative histograms of MV counts vs. control samples from a single individual are also shown (red lines, control; blue lines, experiment). Platelet-free plasma (PFP) from heparin-anticoagulated plasma at (E) 1 week and (F) 4 weeks. PFP from EDTA-anticoagulated plasma at (G) 1 week and (H) 4 weeks. Control samples were collected and centrifuged immediately to isolate MVs (i.e. there was no prior storage of the PFP). Data are expressed as the mean ± standard error of the mean. ^aNo statistically significant difference; ^bP<0.05; ^cP<0.01. MV, microvesicle; EDTA, ethylenediaminetetraacetic acid.