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. 2015 Sep 25;10(6):2093–2101. doi: 10.3892/etm.2015.2780

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Copyright: © Kong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Impact of storage of MVs isolated from EDTA-anticoagulated plasma at 4 or −80°C for different periods. Impact on (A) MV counts and (B) size distribution, respectively. Representative histograms showing the impact of MV storage on MV counts vs. a control sample from the same individual are also presented (red lines, control; blue lines, experiment). Samples were stored at 4°C for (C) 3–4 days or (D) 1 week, and at −80°C for (E) 1 week or (F) 4 weeks. The control sample was collected and centrifuged immediately to isolate MVs, which were not stored. Data are expressed as the mean ± standard error of the mean. ^aNo significance; ^bP<0.05; ^cP<0.01. MV, microvesicle; EDTA, ethylenediaminetetraacetic acid.