Figure 4.

The inhibition of miR-214 contributes to the proliferation of hepatocellular carcinoma SMMC-7721 cells. (A) Cell proliferation ability was determined by MTT assay at 24, 48 and 72 h post-transfection with an inhibitor of miR-214 or control inhibitor. (B) The ability to form colonies was used to detect cell proliferation in SMMC-7721 cells, transfected as indicated in panel A. (C) The cell cycle was determined in SMMC-7721 cells at 48 h post-transfection with the inhibitor of miR-214 or inhibitor-ctrl. The histogram represents the percentage of cells in the G₀-G₁, S and G₂-M cell cycle phases. (D) The expression levels of E2F3, CDK6 and CCND1 were measured by western blotting at 48 h post-transfection with the inhibitor of miR-214 or inhibitor-ctrl. GAPDH was used as a reference control. The data corresponds to the results of 3 independent experiments.*P<0.05 vs. inhibitor-ctrl. E2F3, E2F transcription factor 3; CDK6, cyclin-dependent kinase 6; CCND1, cyclin D1; ctrl, control; OD, optical density.