. 2015 Dec 1;11:292. doi: 10.1186/s12917-015-0609-0

Table 3.

The effect of TC (42 °C for 6 h; posthatch day 28) during 4 time terminals (at the beginning and after 1, 3 and 5 h of TC) on plasma protein pattern and cortisol of broiler chicks subjected to different TM protocols at ED 12–18 (n = 5)

Parameters	Groups	Treatments
Parameters	Groups	Control	TM₁	TM₂	TM₃	TM₄
Total proteins	TN	28.4 ± 0.3 ^aw	37.5 ± 1.1 ^bw	36.0 ± 1.3 ^bw	35.0 ± 1.4 ^bw	37.1 ± 1.4 ^bw
	TC₁	21.9 ± 0.1 ^ax	27.2 ± 1.0 ^bx	27.5 ± 0.9 ^bx	26.6 ± 1.1 ^bx	26.8 ± 1.6 ^bx
	TC₃	22.7 ± 0.2 ^ax	27.4 ± 1.1 ^bx	27.5 ± 1.1 ^bx	26.7 ± 1.0 ^bx	27.5 ± 1.2 ^bx
	TC₅	22.6 ± 1.5 ^ax	29.6 ± 0.6 ^bx	28.0 ± 1.0 ^bx	28.1 ± 1.0 ^bx	28.2 ± 0.8 ^bx
Albumin	TN	17.5 ± 0.6 ^aw	20.2 ± 1.1 ^bw	20.2 ± 1.0 ^bw	20.3 ± 1.2 ^bw	20.3 ± 1.1 ^bw
	TC₁	12.0 ± 0.2 ^ax	15.5 ± 0.5 ^bx	15.4 ± 0.3 ^bx	14.0 ± 0.7 ^bx	14.8 ± 0.4 ^bx
	TC₃	12.1 ± 0.2 ^ax	15.2 ± 0.8 ^bx	15.9 ± 0.7 ^bx	14.7 ± 1.0 ^bx	14.9 ± 1.0 ^bx
	TC₅	12.4 ± 0.1 ^ax	15.1 ± 0.7 ^bx	14.7 ± 0.4 ^bx	14.6 ± 1.7 ^bx	15.1 ± 2.3 ^bx
Corticosterone	TN	0.21 ± 0.03^aw	0.21 ± 0.02 ^aw	0.22 ± 0.01 ^aw	0.22 ± 0.03 ^aw	0.20 ± 0.02 ^aw
	TC₁	0.21 ± 0.00 ^aw	0.20 ± 0.01 ^aw	0.21 ± 0.01 ^aw	0.22 ± 0.05 ^aw	0.21 ± 0.03 ^aw
	TC₃	0.21 ± 0.01 ^aw	0.21 ± 0.11 ^aw	0.21 ± 0.01 ^aw	0.21 ± 0.12 ^aw	0.21 ± 0.01 ^aw
	TC₅	0.22 ± 0.05 ^aw	0.21 ± 0.05 ^aw	0.20 ± 0.01 ^aw	0.21 ± 0.05 ^aw	0.22 ± 0.01 ^aw

Control 37.8 °C, TM ₁ Thermal manipulation at 38.5 °C for 18 h, TM ₂ Thermal manipulation at 39 °C for 18 h, TM ₃ Thermal manipulation at 39.5 °C for 18 h, TM ₄ Thermal manipulation at 40 °C for 18 h, TC Thermal challenged

^a–b within rows, means ± SD with different superscripts differ significantly (P < 0.05)

^w-x Between naïve and TC chicks within a parameter, means ± SD with different superscripts differ significantly (P < 0.05)