Fig. 2.

Effect of CYP1B1 knockdown on cell proliferation, migration and invasion in RCC cell lines. a Knockdown of CYP1B1 levels in RCC cell lines (Caki-1 and 769-P) were determined by real time RT-PCR and Western immunoblot analysis at 48 h after transfection with two different CYP1B1 siRNAs. b Cell viability was analyzed by the MTS cell proliferation assay 0, 24, 48 and 72 h after siRNA treatment. Attenuation of CYP1B1 significantly inhibited cell viability in both cell lines. *, P < 0.05. **, P < 0.01.***, P < 0.001. c Representative images of wound healing assay. After siRNA transfection for 48 h, a wound was formed by scraping and closure of wound measured after 24 h. Attenuation of CYP1B1 significantly inhibited cell migration. **, P < 0.01.***, P < 0.001. d Representative images of invasion assay. Down-regulation of CY1B1 significantly decreases cell invasion. ***, P < 0.001