Fig. 1.

Gene IDs and plant expression cassettes. a Names, accession numbers and amino acid sequence range of PfGAP50 (for further details refer to the methods section). b Schematic drawing of the expression cassettes in the plant binary expression vector pTRAkc (not to scale). SAR: scaffold attachment region; P35SS: Cauliflower mosaic virus 35S promoter with duplicated 35S enhancer region; CHS 5ʹ UTR: 5ʹ untranslated region of the Petroselinum crispum chalcone synthase gene; SP: transit peptide sequence of the murine antibody 24 heavy chain [60]; TP: chloroplast targeting signal from small subunit of RuBisCO from Solanum tuberosum; GAP50: PfGAP50; His₆-tag: six histidine tag for IMAC purification; SEKDEL: ER-retention signal. pA35S: Cauliflower mosaic virus 35S polyadenylation signal. Relevant restriction sites are indicated