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. 2015 Dec 1;15:108. doi: 10.1186/s12896-015-0225-x

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© Beiss et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — SDS-PAGE/immunoblot analysis of plant produced PfGAP50. a: Purification of plastid-targeted PfGAP50 (PfGAP50-cTPH). For purification, 7.0 g infiltrated leaf material were used. Reducing SDS-PAGE (left panel) and immunoblot (right panel). b: Purification of ER-retarded PfGAP50 (PfGAP50-ERH). For purification, 4.5 g infiltrated leaf material were used. Reducing SDS-PAGE (left panel) and immunoblot (right panel). M: Prestained protein marker (Page Ruler Fermentas), 1: 3 μL load (plant extract), 2: 3 μL flow-through, 3: 6 μL elution step 1 (10 mM imidazole), 4: 6 μL elution step 2 (100 mM imidazole), 5: 6 μL elution step 3 (250 mM imidazole). Western blot was detected with rabbit anti-His6 serum and alkaline phosphatase labeled goat anti rabbit serum. c: Plot of mean values and standard deviation of the yields for finally purified (E3) PfGAP50-cTPH and PfGAP50-ERH, determined by densitometric analysis (against BSA equivalents) of SDS-PAGE from three independent replicates (SDS-PAGE shown in additional file 1)