Skip to main content
. 2015 Sep 2;10(5):2899–2904. doi: 10.3892/ol.2015.3665

Figure 1.

Figure 1.

Effects of SAHA on the K562 and K562G CML cell lines. (A) K562 and K562G cells were treated with SAHA (0.5–8 µM) for 48 h. Cell viability was measured using an MTT assay. Data are presented as the mean ± standard deviation of three independent experiments. (B) K562 cells were treated with SAHA (1–4 µM) for 24 h. Western blot analysis was performed, using antibodies against caspase-9, caspase-3, PARP and Ac-histone H3. β-actin was used as a loading control. A representative blot from three independent experiments is shown. (C) K562 cells were treated with SAHA (1–4 µM) for 24 h (upper panel) or with 4 µM SAHA for 3 h, 6 h or 12 h (lower panel). Western blot analysis for Bcr-Abl and p-Bcr-Abl was performed. β-actin was used as a loading control. A representative blot from three independent experiments is shown. SAHA, suberoylanilide hydroxamic acid; CML, chronic myeloid leukemia; Ac-histone, acetylated histone; PARP, poly adenosine diphosphate-ribose polymerase.